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Purity Test results after middle-age and after long-term storage using the Ninhydrin and Ehrlich's reagent methods.](ijerph-09-00869-g002){#ijerph-09-00869-f002} ![Purity Test results after acid precipitation using the Bovine Albumin Protein test.](ijerph-09-00869-g003){#ijerph-09-00869-f003} ijerph-09-00869-t003_Table 3 ###### Pollution Indices (PIs) for each level of the different scenarios for each method. Scenario Pollution Index of E. coli for each method and level ----------- ------------------------------------------------------ ----------- --------- ----------- ----------- ----------- ----------- ---------- --------- ---------- --------- ---------- ---------- ---------- --------- **A1** 0 1.3E+00 1.3E+00 1.2E+00 1.3E+00 1.3E+00 1.3E+00 1.3E+00 1.2E+00 1.3E+00 1.3E+00 1.3E+00 1.3E+00 1.2E+00 1.3E+00 1.3E+00 **B1** 2.0E+00 2.3E+00 2.0E+00 2.0E+00 2.0E+00 2.3E+00 2.3E+00 2.3E+00 2.0E+00 2.0E+00 2.0E+00 2.3E+00 2.3E+00 2.3E+00 2.0E+00 2.0E+00 ========== A2 3.0E+00 4.0E+00 3.0E+00 3.0E+00 3.0E+00 4.0E+00 4.0E+00 4.0E+00 3.0E+00 3.0E+00 3.0E+00 4.0E+00 4.0E+00 4.0E+00 4.0E+00 ========== B2 6.0E+00 8.0E+00 6.0E+00 6.0E+00 6.0E+00 8.0E+00 8.0E+00 8.0E+00 6.0E+00 6.0E+00 6.0E+00 8.0E+00 8.0E+00 8.0E+00 8.0E+00 ========== B3 1.3E+01 1.5E+01 1.3E+01 1.3E+01 1.3E+01 1.5E+01 1.5E+01 1.5E+01 1.3E+01 1.3E+01 1.3E+01 1.5E+01 1.5E+01 1.5E+01 1.5E+01 ========== B4 2.0E+01 2.2E+01 2.0E+01 2.0E+01 2.0E+01 2.2E+01 2.2E+01 2.2E+01 2.0E+01 2.0E+01 2.0E+01 2.2E+01 2.2E+01 2.2E+01 2.2E+01 ========== B5 2.3E+01 2.5E+01 2.3E+01 2.3E+01 2.3E+01 2.5E+01 2.5E+01 2.5E+01 2.3E+01 2.3E+01 2.3E+01 2.5E+01 2.5E+01 2.5E+01 2.5E+01 ========== B6 2.6E+01 2.8E+01 2.6E+01 2.6E+01 2.6E+01 2.8E+01 2.8E+01 2.8E+01 2.6E+01 2.6E+01 2.6E+01 2.8E+01 2.8E+01 2.8E+01 2.8E+01 ========== B7 3.0E+01 3.2E+01 3.0E+01 3.0E+01 3.0E+01 3.2E+01 3.2E+01 3.2E+01 3.0E+01 3.0E+01 3.0E+01 3.2E+01 3.2E+01 3.2E+01 3.2E+01 ijerph-09-00869-t004_Table 4 ###### Pollution Indices (PIs) for each level of the different scenarios for each method. Method --------------------------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- **Bovine Albumin Protein** 2.0E+0 2.3E+0 3.0E+0 4.0E+0 1.3E+1 1.5E+1 2.0E+1 2.3E+1 1.3E+2 1.5E+2 2.0E+2 **Phenol Precipitation** 5.0E+0 6.0E+0 7.0E+0 9.0E+0 6.0E+1 6.5E+1 8.0E+1 9.0E+1 7.0E+2 8.0E+2 9.0E+2 ijerph-09-00869-t005_Table 5 ###### Cumulative volume of water and sewage samples necessary to comply with the proposed pollution indices for different scenarios and methods. Scenario Method ----------- -------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- ------- **B6** 0.4 0.55 0.61 0.63 0.64 0.66 0.68 0.74 0.77 0.82 0.88 0.92 **B5** 1.1 1.27 1.39 1.45 1.49 1.54 1.60 1.77 1.84 2.04 2.23 2.38 ========== B3 4.10 4.85 5.53 6.10 6.68 7.23 8.28 9.37 10.64 12.03 13.50 ========== B4 8.21 9.23 10.10 10.76 11.36 11.92 12.55 13.19 13.84 14.79 15.55 ========== B2 12.40 14.05 14.93 15.74 16.39 17.05 17.79 19.43 20.82 22.25 23.70 ========== B7 15.71 18.40 20.05 21.79 23.47 24.07 25.69 28.31 30.86 33.36 35.83 ========== B3 18.78 22.52 24.32 25.73 27.11 28.44 30.69 34.09 36.44 40.68 43.93 ========== B4 27.07 31.48 34.01 36.31 39.48 41.62 43.85 46.61 50.34 53.76 57.15 ========== B2 36.10 43.45 48.14 51.69 55.19 57.66 60.01 64.20 67.87 71.58 75.11 ========== B7 48.04 57.45 62.63 67.77 72.78 76.71 80.60